Note
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Activation thresholds with LIFE and cuff-like electrodes
This script evaluates the activation threshold of myelinated fibers when stimulated with a cuff electrode and with a LIFE
CUFF - PW = 50us - D_axon 2.0um
Threshold: 399.6µA
CUFF - PW = 50us - D_axon 4.0um
Threshold: 160.0µA
CUFF - PW = 50us - D_axon 6.0um
Threshold: 90.5µA
CUFF - PW = 50us - D_axon 8.0um
Threshold: 67.1µA
CUFF - PW = 50us - D_axon 10.0um
NRV WARNING: [18400.00390625 100. 0. ] not found in mesh, value of [18400. 100. 0.] reused
Threshold: 53.8µA
CUFF - PW = 50us - D_axon 12.0um
Threshold: 49.1µA
CUFF - PW = 50us - D_axon 14.0um
Threshold: 46.4µA
CUFF - PW = 50us - D_axon 16.0um
NRV WARNING: [21000.00390625 100. 0. ] not found in mesh, value of [21000. 100. 0.] reused
Threshold: 43.8µA
CUFF - PW = 50us - D_axon 18.0um
Threshold: 41.7µA
CUFF - PW = 50us - D_axon 20.0um
Threshold: 40.3µA
LIFE - PW = 50us - D_axon 2.0um
Threshold: 76.5µA
LIFE - PW = 50us - D_axon 4.0um
Threshold: 37.1µA
LIFE - PW = 50us - D_axon 6.0um
Threshold: 23.4µA
LIFE - PW = 50us - D_axon 8.0um
Threshold: 17.9µA
LIFE - PW = 50us - D_axon 10.0um
NRV WARNING: [12650.00488281 100. 0. ] not found in mesh, value of [12650. 100. 0.] reused
Threshold: 15.7µA
LIFE - PW = 50us - D_axon 12.0um
Threshold: 14.7µA
LIFE - PW = 50us - D_axon 14.0um
Threshold: 14.1µA
LIFE - PW = 50us - D_axon 16.0um
Threshold: 13.7µA
LIFE - PW = 50us - D_axon 18.0um
Threshold: 13.2µA
LIFE - PW = 50us - D_axon 20.0um
Threshold: 13.0µA
import nrv
import matplotlib.pyplot as plt
import numpy as np
import nrv
import matplotlib.pyplot as plt
import numpy as np
nrv.parameters.set_nrv_verbosity(2)
#Update function for the binary search
def cathodic_pulse_update(axon,amp, pw, elec_id = 0, start_p = 1):
stim = nrv.stimulus()
stim.pulse(start_p,-amp,pw)
axon.change_stimulus_from_electrode(elec_id, stim)
if __name__ == '__main__':
#Stimulus definition
t_inter = 0
cath_an_ratio = 0
anod_first = False
pw = 50e-3 #stimulation PW is 50µs
#binary search parameters
amp_max = 600 #maximum stimulation amplitude, in µs
amp_tol = 5 #binary search tolerance, in %
#Axon ranges from 1µm to 20µm
d_min = 2
d_max = 20
n_diam = 10
diam_list = np.round(np.linspace(d_min,d_max,num=n_diam))
model= 'MRG'
n_node = 20 #20 Node of Ranvier for each axon
# axon location
z_axon = 0 # axon z position, in [um]
y_axon = 100 # axon z position, in [um]
##### electrodes definition
contact_width=500
contact_thickness=100
insulator_width=1000
insulator_thickness=500
### Simulation box size
Outer_D = 6 # in in [mm]
#### Nerve and fascicle geometry
Nerve_D = 1000 # in [um]
fasc_geom = nrv.create_cshape(diameter=800) # in [um]
perineurium_thickeness = 25 # in [um]
#dummy useless stimulus
start = 1
I_cathod = 10
I_anod = 0
T_cathod = pw
T_inter = t_inter
stim1 = nrv.stimulus()
stim1.biphasic_pulse(start, I_cathod, T_cathod, I_anod, T_inter)
thr_cuff = []
for diam in diam_list:
L=nrv.get_length_from_nodes(diam,n_node)
print(f"CUFF - PW = {int(pw*1e3)}us - D_axon {diam}um")
#set the FEM parameters
extra_stim = nrv.FEM_stimulation()
extra_stim.reshape_outerBox(Outer_D)
extra_stim.reshape_nerve(Nerve_D, L)
extra_stim.reshape_fascicle(fasc_geom)
#axon creation
axon1 = nrv.myelinated(y_axon,z_axon,diam,L,rec='nodes',model=model)
n_node = len(axon1.x_nodes)
x_elec = axon1.x_nodes[(n_node//2)] # electrode x position, in [um]
elec_1 = nrv.CUFF_electrode('CUFF_1', contact_length=contact_width,\
contact_thickness=contact_thickness, insulator_length=insulator_width,\
insulator_thickness=insulator_thickness, x_center=x_elec)
# extracellular stimulation setup
extra_stim.add_electrode(elec_1, stim1)
axon1.attach_extracellular_stimulation(extra_stim)
axon1.get_electrodes_footprints_on_axon()
start_p = 1
arg_stim = {'pw':pw, 'elec_id':0, 'start_p':start_p} #parameters for the waveforms
new_thr = nrv.axon_AP_threshold(axon = axon1,amp_max = amp_max,
update_func = cathodic_pulse_update, args_update=arg_stim)
print(f"Threshold: {np.round(new_thr,1)}µA")
thr_cuff.append(new_thr)
del extra_stim,axon1 #to prevent meshing error (known bug)
#binary search parameters
amp_max = 150 #maximum stimulation amplitude, in µs
#LIFE
LIFE_length = 1000 #electrode active site length
y_elect = 0
z_elect = 0
D_1 = 25 #electrode diam
thr_LIFE = []
for diam in diam_list:
L=nrv.get_length_from_nodes(diam,n_node)
print(f"LIFE - PW = {int(pw*1e3)}us - D_axon {diam}um")
#set the FEM parameters
extra_stim = nrv.FEM_stimulation()
extra_stim.reshape_outerBox(Outer_D)
extra_stim.reshape_nerve(Nerve_D, L)
extra_stim.reshape_fascicle(fasc_geom)
#axon creation
axon1 = nrv.myelinated(y_axon,z_axon,diam,L,rec='nodes',model=model)
n_node = len(axon1.x_nodes)
x_elec = axon1.x_nodes[n_node//2] # electrode y position, in [um]
y_c = 0
x_1_offset = x_elec - (LIFE_length/2)
LIFE = nrv.LIFE_electrode('LIFE_1', D_1, LIFE_length, x_1_offset, y_elect, z_elect)
# extracellular stimulation setup
extra_stim.add_electrode(LIFE, stim1)
axon1.attach_extracellular_stimulation(extra_stim)
axon1.get_electrodes_footprints_on_axon()
start_p = 1
arg_stim = {'pw':pw, 'elec_id':0, 'start_p':start_p} #parameters for the waveforms
new_thr = nrv.axon_AP_threshold(axon = axon1,amp_max = amp_max,
update_func = cathodic_pulse_update, args_update=arg_stim)
print(f"Threshold: {np.round(new_thr,1)}µA")
thr_LIFE.append(new_thr)
del extra_stim,axon1 #to prevent meshing error (known bug)
fig,ax = plt.subplots(1)
ax.semilogy(diam_list,thr_LIFE,label ='LIFE')
ax.semilogy(diam_list,thr_cuff,label ='cuff')
ax.legend()
ax.set_xlabel("Axon diameter (µm)")
ax.set_ylabel("Activation threshold (µA)")
fig.tight_layout()
plt.show()
Total running time of the script: (4 minutes 13.949 seconds)